WP 2: Genomic determinants of the inflammatory response

Exacta ChIP complex

Objectives

The objective of this work package is to map binding sites for inflammatory transcription factors (TFs) genome-wide and understand:

1- rules and principles describing their usage;
2- the direct transcriptional effects of their occupancy.

Specific aims:

  • Understanding the contribution of the main inflammatory TFs to the gene expression program triggered by inflammatory stimuli;
  • Discriminating direct versus indirect TF targets by mapping their binding sites in the genome;
  • Discriminating binding events mediated by direct interaction with binding sites from binding events mediated by interactions with DNA-bound TFs;
  • Devising computational methods to analyze and quantify results of high-throughput measurements of TF binding to DNA;
  • Characterizing TF affinities to DNA sequences in vivo;
  • Quantifying contribution of TFs to the transcriptional response of genes.